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elisa kit  (Elabscience Biotechnology)


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    Elabscience Biotechnology elisa kit
    Elisa Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+c5a+elisa+kit/Rat+C5a+(Complement+Component+5a)+ELISA+Kit/pm40855320-97-69-71
    Average 93 stars, based on 8 article reviews
    elisa kit - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Effect of a C5a receptor antagonist on macrophage function in an intestinal transplant rat model.
    Article Snippet: Background: C5a promotes alloreactivity via the C5a receptor 1 (C5aR1) on immune cells, but this has not been confirmed in the case of small intestine transplantation immunity.. In the present study, we examined the effect of C5aR1 antagonist (PMX53) on macrophage function in small intestinal transplantation.. Methods: The model was created by heterotopic intestinal transplantation using donor Dark Agouti and recipient Lewis rats.

    Article Title: C5a aggravates dysfunction of the articular cartilage and synovial fluid in rats with knee joint immobilization
    Article Snippet: .. The Rat IL-1β ELISA kit (cat. no. E-EL-R0012c), Rat C5a ELISA kit (cat. no. E-EL-R0257c), Rat TNF-α ELISA kit (cat. no. E-EL-R0019c) and Rat IL-17A ELISA Kit (cat. no. E-EL-R0566c; all Elabscience, Houston, TX, USA) were used. .. The absorbance was measured at 450 nm using a microplate spectrophotometer (Multiskan GO; Thermo Fisher Scientific, Inc.).

    Article Title: The Novel C5aR Antagonist DF3016A Protects Neurons Against Ischemic Neuroinflammatory Injury.
    Article Snippet: .. Quantitative measurements of cellular proteins (C5a, TNF-α, IL-1β, and IL-6) in cell culture supernatants were determined by ELISA methods using Rat IL-6, Mouse TNFα, Rat IL-1β PicoKineTM ELISA Kits (Boster Biological Technology) and Rat C5a ELISA Kit (Elabscience Biotechnology Inc.) according to the manufacturer’s instructions. ..

    Article Title: The Novel C5aR Antagonist DF3016A Protects Neurons Against Ischemic Neuroinflammatory Injury
    Article Snippet: .. Quantitative measurements of cellular proteins (C5a, TNF-α, IL-1β, and IL-6) in cell culture supernatants were determined by ELISA methods using Rat IL-6, Mouse TNFα, Rat IL-1β PicoKine TM ELISA Kits (Boster Biological Technology) and Rat C5a ELISA Kit (Elabscience Biotechnology Inc.) according to the manufacturer’s instructions. ..

    Article Title: C5a aggravates dysfunction of the articular cartilage and synovial fluid in rats with knee joint immobilization.
    Article Snippet: .. The Rat IL-1β ELISA kit (cat. no. E-EL-R0012c), Rat C5a ELISA kit (cat. no. E-EL-R0257c), Rat TNF-α ELISA kit (cat. no. E-EL-R0019c) and Rat IL-17A ELISA Kit (cat. no. E-EL-R0566c; all Elabscience, Houston, TX, USA) were used. .. The absorbance was measured at 450 nm using a microplate spectrophotometer (Multiskan GO; Thermo Fisher Scientific, Inc.).

    Cell Culture:

    Article Title: The Novel C5aR Antagonist DF3016A Protects Neurons Against Ischemic Neuroinflammatory Injury.
    Article Snippet: .. Quantitative measurements of cellular proteins (C5a, TNF-α, IL-1β, and IL-6) in cell culture supernatants were determined by ELISA methods using Rat IL-6, Mouse TNFα, Rat IL-1β PicoKineTM ELISA Kits (Boster Biological Technology) and Rat C5a ELISA Kit (Elabscience Biotechnology Inc.) according to the manufacturer’s instructions. ..

    Article Title: The Novel C5aR Antagonist DF3016A Protects Neurons Against Ischemic Neuroinflammatory Injury
    Article Snippet: .. Quantitative measurements of cellular proteins (C5a, TNF-α, IL-1β, and IL-6) in cell culture supernatants were determined by ELISA methods using Rat IL-6, Mouse TNFα, Rat IL-1β PicoKine TM ELISA Kits (Boster Biological Technology) and Rat C5a ELISA Kit (Elabscience Biotechnology Inc.) according to the manufacturer’s instructions. ..



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    Effect of repeated oral administration of K/Na citrate on the concentrations of complement component anaphylatoxins C3a and C5a in rat plasma. ( A ) C3a and ( B ) C5a rat plasma complement component levels on day 22 in rat PTX-treated model measured using an enzyme-linked immunosorbent assay (ELISA) kit. Data are presented as mean ± SEM ( n = 4–8). * p < 0.05 (one-way ANOVA with post hoc Tukey–Kramer multiple comparison tests).

    Journal: International Journal of Molecular Sciences

    Article Title: Potassium/Sodium Citrate Attenuates Paclitaxel-Induced Peripheral Neuropathy

    doi: 10.3390/ijms26073329

    Figure Lengend Snippet: Effect of repeated oral administration of K/Na citrate on the concentrations of complement component anaphylatoxins C3a and C5a in rat plasma. ( A ) C3a and ( B ) C5a rat plasma complement component levels on day 22 in rat PTX-treated model measured using an enzyme-linked immunosorbent assay (ELISA) kit. Data are presented as mean ± SEM ( n = 4–8). * p < 0.05 (one-way ANOVA with post hoc Tukey–Kramer multiple comparison tests).

    Article Snippet: Plasma C3a and C5a levels were measured using the Rat Complement Fragment 3a (C3a) ELISA Kit (CSB-E08510r, Cusabio Technology LLC, Houston, TX, USA) and the Rat Complement C5a ELISA Kit (LS-F34383-1, Lifespan Biosciences, Inc., Seattle, WA, USA), respectively, following the manufacturer’s instructions.

    Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Comparison

    Time course of the C5a/C5aR1 axis, expression in rat kidney sections, C5a concentration in rat plasma and numbers of renal macrophages. The left kidneys were harvested after I/R at the following time points: 10 min, 6 h, 24 h, 3 d, 5 d and 8 w. The healthy right kidney was nephrectomized one week before and served as a control. mRNA was isolated from fresh-frozen rat kidney tissue. C5a was measured in rat plasma using an ELISA ( A ). Plasma C5a concentrations were correlated with serum urea ( B ). Expression of C5ar1 ( C ) and C5 ( D ). mRNA was analyzed at different time points post-perfusion. C5ar1 mRNA expression correlated with the expression of the macrophage marker Cd68 ( E ), while no correlation was found between Cd68 expression and plasma C5a levels ( F ). Immunohistochemistry was used for the evaluation of CD163-positive ( G ) and CD68-positive macrophages in kidney sections ( H ) (ns = not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

    Journal: Life

    Article Title: Macrophage-Induced Pro-Fibrotic Gene Expression in Tubular Cells after Ischemia/Reperfusion Is Paralleled but Not Directly Mediated by C5a/C5aR1 Signaling

    doi: 10.3390/life14081031

    Figure Lengend Snippet: Time course of the C5a/C5aR1 axis, expression in rat kidney sections, C5a concentration in rat plasma and numbers of renal macrophages. The left kidneys were harvested after I/R at the following time points: 10 min, 6 h, 24 h, 3 d, 5 d and 8 w. The healthy right kidney was nephrectomized one week before and served as a control. mRNA was isolated from fresh-frozen rat kidney tissue. C5a was measured in rat plasma using an ELISA ( A ). Plasma C5a concentrations were correlated with serum urea ( B ). Expression of C5ar1 ( C ) and C5 ( D ). mRNA was analyzed at different time points post-perfusion. C5ar1 mRNA expression correlated with the expression of the macrophage marker Cd68 ( E ), while no correlation was found between Cd68 expression and plasma C5a levels ( F ). Immunohistochemistry was used for the evaluation of CD163-positive ( G ) and CD68-positive macrophages in kidney sections ( H ) (ns = not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

    Article Snippet: Rat plasma samples collected at 10 min, 6 h, 24 h, 3 days, 5 days and 8 weeks after I/R were analyzed for C5a using a rat complement component C5a ELISA kit (NBP2-82137, Novus Biologicals, Centennial, CO, USA) following the manufacturer’s instructions and using a Synergy microplate reader and Gen5 software (BioTek Instruments GmbH, Friedrichshall, Germany).

    Techniques: Expressing, Concentration Assay, Clinical Proteomics, Control, Isolation, Enzyme-linked Immunosorbent Assay, Marker, Immunohistochemistry

    Expression of pro-fibrotic genes in human tubular cells and supernatant concentration of TGF-ß after co-cultivation under normoxic or hypoxic conditions with or without C5a stimulation. mRNA isolated from human proximal tubular cells (HPTC) after a 24 h incubation (21% O 2 or 1% O 2 ) in co-culture with THP-1 monocytes differentiated to macrophages and subsequent stimulation with 50 nM C5a for 24 h. Expression of FGF2 ( A ), SNAI1 ( B ), ACTA2 ( C ), VIM ( D ) and TGFB1 ( F ). Supernatant was collected and TGF-ß concentration was determined by an ELISA ( E ) (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; § p < 0.05 vs. Ctrl.).

    Journal: Life

    Article Title: Macrophage-Induced Pro-Fibrotic Gene Expression in Tubular Cells after Ischemia/Reperfusion Is Paralleled but Not Directly Mediated by C5a/C5aR1 Signaling

    doi: 10.3390/life14081031

    Figure Lengend Snippet: Expression of pro-fibrotic genes in human tubular cells and supernatant concentration of TGF-ß after co-cultivation under normoxic or hypoxic conditions with or without C5a stimulation. mRNA isolated from human proximal tubular cells (HPTC) after a 24 h incubation (21% O 2 or 1% O 2 ) in co-culture with THP-1 monocytes differentiated to macrophages and subsequent stimulation with 50 nM C5a for 24 h. Expression of FGF2 ( A ), SNAI1 ( B ), ACTA2 ( C ), VIM ( D ) and TGFB1 ( F ). Supernatant was collected and TGF-ß concentration was determined by an ELISA ( E ) (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; § p < 0.05 vs. Ctrl.).

    Article Snippet: Rat plasma samples collected at 10 min, 6 h, 24 h, 3 days, 5 days and 8 weeks after I/R were analyzed for C5a using a rat complement component C5a ELISA kit (NBP2-82137, Novus Biologicals, Centennial, CO, USA) following the manufacturer’s instructions and using a Synergy microplate reader and Gen5 software (BioTek Instruments GmbH, Friedrichshall, Germany).

    Techniques: Expressing, Concentration Assay, Isolation, Incubation, Co-Culture Assay, Enzyme-linked Immunosorbent Assay